STUDIES ON NUCLEAR STRUCTURE AND FUNCTION IN TETRAHYMENA PYRIFORMIS
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چکیده
منابع مشابه
Studies on Nuclear Structure and Function in Tetrahymena Pyriformis
This report describes a rapid, efficient method for isolating macronuclei from Tetrahymena. The macronuclear fraction contains only small amounts of micronuclear material and little detectable whole cell or cytoplasmic contamination. A method is also described for preparing a "micronuclear fraction" which contains 20-40 micronuclei for every macronucleus present. Electron microscope observation...
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A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes commonly associated with this organism. Analysis of the membrane lipids of these fractions reveals ...
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Meyerhof, 0. (1945). J. biol. Chem. 157, 105. Meyerhof, 0. & Kaplan, A. (1951). Arch. Biochem. Biophy8. 38, 282. Meyerhof, 0. & Oesper, P. (1947). J. biol. Chem. 170, 1. Meyerhof, 0. & Wilson, J. R. (1949). J. biol. Chem. 180, 575. Nelson, N. (1944). J. biol. Chem. 153, 373. Nilsson, R. & Alm, F. (1941). Biochem. Z. 308, 262. Prankerd, T. A. J. & Altman, K. I. (1954). Biochem. J. 58, 622. Roe, ...
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Increasing concentrations of dimethyl sulphoxide (DMSO) affect vacuole formation in Tetrahymena, as measured quantitatively by the uptake of carmine particles. The rate of vacuole formation decreased to about 50% of the control value in 5 0 % DMSO (v/v) and to zero in 7 5 % . At the latter concentration, the inhibition was expressed immediately; however, the effect of i-h exposure was reversibl...
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Histones were extracted from isolated macroand micronuclear fractions and from nucleohistone fibers which were prepared from the isolated macronuclear fraction . Analysis of these histones by polyacrylamide gel electrophoresis indicated that there are electrophoretic differences between the histones of macroand micronuclei . THE JOURNAL OF CELL BIOLOGY . VOLUME 47, 1970 . pages 63 1-636 contain...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1970
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.47.3.619